ABSTRACT
Cherubism is a rare non-neoplastic disease of the bone characterized by bilateral painless enlargement of the jaws giving a cherubic appearance to the patient. It is an autosomal dominant disorder but may occur sporadically. In this paper, we have tried to explain about the clinical, radiological and histopathologic features of cherubism by presenting a case study. A case study of a 7 year old male patient, who first presented with mandibular bilateral swellings to our department in 1998, has been presented. In-depth clinical, radiological and histopathologic examination was done. An extensive long-term follow-up till 11 years was maintained. When presented for the first time, radiological investigation showed characteristic multilocular lytic lesions of the mandible bilaterally. Histopathological examination of the biopsy specimen showed proliferating fibrous connective tissue interspersed by multinucleated giant cells. It was diagnosed as a case nonfamilial cherubism. Follow-up after 5 years showed involvement of the maxilla as well, which was then corrected by surgical methods. Upon follow up 11 years after the first presentation, it was seen that the lesion was regressing by itself and there was improvement in facial contour. The natural course of Cherubism through its progression, stabilization and involution of the disease after puberty, has been highlighted in this case. More, in-depth studies to understand the nature and the pathogenesis of this condition better are required.
Subject(s)
Biopsy , Cherubism/diagnosis , Cherubism/pathology , Child , Follow-Up Studies , Histocytochemistry , Humans , Jaw/pathology , Jaw/diagnostic imaging , Male , Maxilla/pathology , Maxilla/diagnostic imaging , Microscopy , Tomography, X-Ray ComputedABSTRACT
Post-marketing stability testing of sulphacetamide ophthalmic solutions marketed in Pakistan was undertaken. Nine commercial batches of the drug belonging to six brands with different manufacturing dates covering the shelf-life were selected. The samples were collected from the local drug stores in two different cities namely D.I. Khan and Karachi, which differ in their climatic conditions. The analysis included pH measurement and quantitative determination of the active component sulphacetamide and its degradation product sulphanilamide by HPLC method. All the samples complied with pH limit specified by British Pharmacopoeia. However, only two samples from DI. Khan and four samples from Karachi passed the sulphacetamide assay test. The levels of hydrolytic product sulphanilamide varied between 1.5-7.3% for the samples from DI. khan and 1.02-5.2% for the samples collected from Karachi. It was concluded that ageing and storage conditions had deleterious effect on the stability of those ophthalmic solutions. The higher degradation of suiphacetamide in the samples collected from DI. Khan is attributed to the relatively higher temperature in this area as compared to Karachi
Subject(s)
Product Surveillance, Postmarketing , Drug Stability , Ophthalmic Solutions , Chromatography, High Pressure Liquid , SodiumABSTRACT
Post-marketing stability studies were conducted on different commercial acetaminophen paediatric preparations collected from the cities of Dera Ismail Khan and Karachi, Pakistan, which differ in their climatic conditions. A properly validated HPLC method developed in our laboratory was used. The mobile phase consisting of 0.05M phosphate buffer: methanol:hexane-1-sulfonic acid sodium salt in the ratio 850: 150: 2 [v/v/w], pH adjusted to 3, was pumped at1.5 mI/mm flow. The separation was achieved on a reverse phase C18micro Bondapack 3.9 i.d. x 300 trim column and the aetection wave length was 245 nm. It was concluded that due to warmer climatic conditions the levels of hydrolytic product 4-aminophenol were higher in samples collected from DI. Khan as compared to the samples from Karachi